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Man-BatMan-Bat (Kirk Langstrom) was a former scientist who tested a formula on himself to create a new species, and transformed into a giant bat creature. He is powerful, and can fly. He also has the ability to emit sonic blasts that can not only break glass, but can also disorient or destroy enemies.
Dr. Victor Fries) was a cryogenist until his experiments went terribly wrong when an accident occurred. Mr. Freeze uses frozen weaponry and must wear a refrigerated ice-suit in order to survive. He can go in freezing gasses and has Super-Strength. His weapon of choice is a freeze gun that can temporarily freeze others.
- Former district-attorney Two-Face (Harvey Dent) is a villain with a split personality. His every decision is dictated by a double-headed coin, scarred on one side, which he flips to decide if he'll act for good, or evil. Two-Face has 2 pistols and can use bombs.
Matt Hagen was transformed into the monstrous Clayface by a pool of radioactive protoplasm. He now possesses super-strength and can change his claylike body into any form. Clayface fights with his hands and has Super-Strength.
Pamela Lillian Isley) adores plants and uses her plant-based toxins in her criminal activities. She often teams up with fellow villain Harley Quinn. She fights with plant-bombs and can go on tightrope and acrobat points. She can go in Toxins.
Oswald Chesterfield Cobblepot) is a devious crime-boss who is seldom seen without one of his trick-umbrellas that shoot bullets. The Penguin uses his nightclub, the Iceberg Lounge, as a front for his criminal activities. he can glide, use tight-ropes, and use travel chutes.
Selina Kyle) is an accomplished cat-burglar with a taste for luxury. She is extremely agile and acrobatic. She uses a whip.
Waylon Jones) has a medical condition that warped his body into a massive crocodile-like form. He possesses super-strength and is immune to toxins. He fights with his hands.
Mad Hatter (Jervis Tetch) was inspired by the lunacy of Alice's Adventures Of Wonderland to commit crimes. He uses his mind-control technology to bend people to his will, and is never seen without a large and fantastic hat.
Dr. Harleen Quinzel was the Joker's psychiatrist at Arkham Asylum...until she fell in love with him and reinvented herself as his madcap sidekick Harley Quinn. She fights with a cork-gun and a hammer. She can go on acrobat poles and can go on tightropes.
Killer Moth"Killer Moth" a dangerous villain attracted to light